Fig. 6

Representative images of sites with lowest classification accuracy in the decision tree model. a-d: Invasive lobular carcinoma and lobules in confocal fluorescence images. e-h: Nuclei segmented by identifying maximally stable extremal regions (MSER) are false-colored green and overlaid onto the raw confocal fluorescence image. i-l: Histologic slides with hematoxylin and eosin (H&E) staining show similar histology to confocal images in (a-e). Slides were prepared with the same specimens from which confocal images were acquired. a, e: A true-positive invasive lobular carcinoma (ILC) site; (b, f): false-negative ILC site; (c, g): true-negative lobules; and (d, h): false-positive lobules. Scale bar is 100 μm