Fig. 3

Representative confocal images of normal, non-hyperplastic ducts (a), hyperplastic ducts (b), and ductal carcinoma in situ (c) analyzed with the nuclear segmentation algorithm (middle row) and with the ductal segmentation algorithm (bottom row). d-f: Nuclei segmented by identifying maximally stable extremal regions (MSER) are false colored green and overlaid onto the raw confocal fluorescence image. g-i: Breast ducts segmented using the ductal segmentation algorithm. j-l: Histologic slides with H&E staining show similar histology to confocal images in a-e. Slides were prepared with the same specimens from which confocal images were acquired. Scale bar is 100 µm