Fig. 5

Py230 cells differentiate to form p53-positive mesenchymal cells. (a) Py230 MaCSCs treated with 0.1 % DMSO undergo epithelial to mesenchymal transition. (b) Flow cytometry profiles of a DMSO-treated Py230 clone showing the transition over time from a luminal to a claudin-low phenotype. (c) A third generation Py230 tumor showing clonal evolution to form an invasive p53-positive tumor extension. (d) Western blot of tumor lysates. Lane 1: tumor from early passage Py230 cells, lane 2: basal-like tumor from Py117, a cell line cloned from a third generation in vivo passaged Py230 tumor, lane 3: claudin-low tumor from Py15-4, a cell line clone from a third generation in vivo passaged Py230 tumor. (e) CD24, CD44 FACS profile of Py230 and Py15-4 clonal cell lines. (f) Karyotype of clonal luminal cell line Py230 and clonal claudin-low cell lines Py15-4 and Py16-1. (g) Growth curves of Py230 luminal cell line and Py15-4 and Py16-1 claudin-low cell lines. Data are means ± SEM of triplicate cultures. Both claudin-low cell lines were derived from Py230 cells