Fig. 4
From: Multipotent luminal mammary cancer stem cells model tumor heterogeneity
During tumor progression Py230 cells undergo changes that upregulate nuclear p53 expression. (a) Tumor latency of Py230 tumors from first generation tumor cell injections (T1, blue) compared with Py230 tumors from third generation injections (T3, red). (b) Karyotyping of the Py230 cell line (Py230) and Py230 first generation tumors from independent animals (T1). Cells isolated from tumors of subsequent generations (T2, T3) undergo changes that result in aneuploidy. (c) Expression profiling of tumors from various generations of Py230 injections compared with spontaneous PyVmT tumors. (d) IHC staining for p53 expression in the different Py230 tumor subtypes