Fig. 2

Py230 mammary cancer stem cells recapitulate spontaneous luminal tumorigenesis and metastasis. a-f, H&E stained sections of Py230 MIN (a), tumor (b) and metastasis (c) compared with spontaneous PyVmT MIN, (d), tumor (e) and metastasis (f). Scale bar = 50 μm. g-j Whole mounts of normal mammary gland (NMG), pre-lactating mammary gland (PLMG), PyVmT MIN and a 10-day outgrowth of Py230 tumor cells in the cleared mammary fat pad. Scale bar = 100 μm. k-r, Representative dot plots of NMG (k,o), PLMG (l,p), PyVmT MIN (m,q) and Py230 tumor (n,r) labeled with CD24 and CD49f (k-n) or CD24 and CD29 (o-r)