Fig. 6
From: Targeting lysyl oxidase for molecular imaging in breast cancer
Flow cytometry of lysyl oxidase (LOX) in EMT-6 cells cultured under (a, c) normoxic conditions versus (b, d) hypoxic conditions. Cells were treated with primary anti-LOX antibody, followed by (a, b) staining with Alexa Fluor 488–labeled secondary antibody or (c, d) treatment with fluorescein isothiocyanate (FITC)-Ava-GGGDPKGGGGG-NH2. The x- and y-axes of each graph show the fluorescence intensity and the number of cells, respectively. Representative results of three experiments are shown. The black open histogram depicts unstained cells. Values in each panel are the ratio of tumor volume in control (C) versus treated (T) mice calculated based on the mean fluorescence intensity of blue closed (T: LOX expression detected with anti-LOX antibody and FITC-Ava-GGGDPKGGGGG-NH2, respectively) and gray open (C: only Alexa Fluor 488–labeled secondary antibody control and GGGDPKGGGGG-NH2 without FITC-labeled control, respectively) histograms (n = 3)