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Fig. 2 | Breast Cancer Research

Fig. 2

From: Targeting IL13Ralpha2 activates STAT6-TP63 pathway to suppress breast cancer lung metastasis

Fig. 2

Knockdown of IL13Rα2 enhances IL-13-mediated STAT6 phosphorylation. a MIV cells were treated with 20 ng/ml IL-13 for 30 min, and Western blot analysis was performed with whole cell lysates to assess phosphorylation levels of STAT1, STAT3, STAT5, and STAT6. b Western blot (i) and real-time polymerase chain reaction (ii) were performed in MIV cells stably transduced with shSCR or shIL13Rα2 lentiviral vectors expressing two different small hairpin RNA oligos (#1 and #2) to measure shIL13Rα2 protein and mRNA levels, respectively. c MIV-shSCR and MIV-shIL13Rα2#2 cells were treated with 1 ng/ml IL-13 for 30 min, and Western blot analysis was performed with whole cell lysates to assess phosphorylation levels of STAT6 (Y641) and STAT3 (Y705 and S727). Total STAT6 and α-tubulin protein levels were detected as loading controls. d SUM159-shSCR and SUM159- shIL13Rα2#2 cells were treated with 1 ng/ml IL-13 for 30 min, and Western blot analysis was performed with whole cell lysates to assess phosphorylation levels of STAT6 (Y641). Total STAT6 and GAPDH protein levels were detected as loading controls. IL-13 interleukin-13, IL13Rα2 interleukin-13 receptor alpha 2, shIL13Rα2 small hairpin RNA against interleukin-13 receptor alpha 2, shSCR scrambled small hairpin RNA, STAT signal transducer and activator of transcription

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