Fig. 5

CD24⁺ cells differentiate and give rise to CD24⁻ cells in vivo. a Experimental strategy scheme. CD24⁻ or CD24⁺ cells were injected into the mammary fat pads; after 3 weeks, tumors were harvested and CD24 expression was evaluated. b CD24 expression in cancer cells from mammary tumors was determined by fluorescence-activated cell sorting analysis (upper panel), and CD24⁻ and CD24⁺ from CD24⁺ inoculated tumors were sorted into a pure population (lower panel). c Different phenotypes for each group shown on phase-contrast bright field images of cells grown in adherent culture