Fig. 4

Relative frequency of spleen lymphocyte subsets in IL-15-deficient and IL-15-proficient, control and vaccinated NeuT mice. Splenocytes were analyzed by flow cytometry in control and vaccinated mice. The percentage of B220+, CD3+, CD4+, CD8+ and Nkp46 and DX5 double-positive cells is reported. Significance of comparisons (Student’s t test): ^* p <0.05, IL15KO/NeuT versus NeuT mice; ^# p <0.05, vaccinated versus control mice within the same strain; ^§ p <0.05, 15- versus 42-week-old mice within the same strain. Mean ± SEM is shown (three to six mice per group). IL15KO/NeuT BALB/c mice transgenic for rat HER2/neu oncogene and knocked out for IL-15, NeuT BALB/c mice transgenic for rat HER2/neu oncogene, SEM standard error of the mean