Fig. 6
Ezh2 mediates repression of mammary epithelial miRNAs. MiR-34b, miR-204 and miR-218 are expressed highly in the MaSC/basal subset. a Track files or read coverage graphs for H3K4me3 and H3K27me3 marks present in the 3 kb upstream region of miR-34b (top panel), miR-204 (middle panel) and miR-218 (bottom panel) in each epithelial subset. Y-axes show fragments per million on the scale 0–10. b Bar graphs represent quantitative RT-PCR analysis for mammary epithelial cells isolated from control (Ezh2f/+) or Ezh2-deficient (MMTV-cre;Ezh2f/f) mammary glands. Expression of the MaSC/basal-specific miRNAs miR-34b, miR-204 and miR-218 is upregulated in luminal cells of Ezh2-deficient samples compared to littermate controls. Data was normalized against U6 small RNA. n = 3 independent biological samples; error bars show SEM. LP luminal progenitor, ML mature luminal, MS MaSC/basal