Fig. 2

Inverse correlation between differentially expressed miRNAs in specific subpopulations and their transcriptomes. Lineage-specific miRNAs are conserved between mouse and human mammary tissue. a Schematic representation of Rotation Gene Set Test (ROAST) analysis [29]. Mouse and human Taqman probes were matched by miRNA symbols obtained from the miRNA database (miRBase) and TargetScan was used to relate miRNAs to target mRNAs. ROAST tests were performed to detect miRNAs that are most negatively correlated with their target mRNAs. b Barcode plots showing the expression patterns of the mouse and human mRNA targets of the conserved luminal-specific miR-200b. Genes are ranked in terms of relative expression from highest in MaSC/basal cells (MS) to highest in luminal cells. Target genes are marked with vertical bars and the worm shows relative enrichment. The target genes tend to be less highly expressed in the luminal than the MaSC/basal subset. c Predicted target genes for the top 20 conserved differentially expressed miRNAs in the two major populations. DE differentially expressed, miRNA miR microRNA, Lum combined luminal progenitor and mature luminal cells