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Fig. 5 | Breast Cancer Research

Fig. 5

From: Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-integrin signaling pathway in tumor cells

Fig. 5

β1-integrin mediates the effect of cancer-associated fibroblasts on breast cancer cell migration. Cell migratory capacity was assessed in Transwell assays and wound healing assay. (a) In vitro Transwell assays were performed using cancer-associated fibroblast (CAF)-conditioned medium (CM) or normal medium with or without the addition of TAM (1 μM). (b) The number of cells with indicated pretreatment that migrated toward the lower wells of the transwell containing CM was counted. Each experiment was repeated at least three times. *P < 0.05. (c) The capacity of cells to migrate to fill a scratched area devoid of cells was assessed in co-cultures of breast cancer cells and CAFs. Before co-culture, breast cancer cells were infected with lentivirus carrying a green fluorescent protein reporter gene to distinguish CAFs under a fluorescence microscope. Fluorescence photomicrographs revealed that MCF-7R-sh/Vec showed enhanced cell migration ability into the scratched area (arrows) when compared with MCF-7R-sh/ITGB1. Ctrl, control; ITGB1, β1-integrin; MCF-7R-sh/ITGB1, MCF-7R cells infected with lentivirus vector targeting β1-integrin; MCF-7R-sh/Vec, MCF-7R cells infected with negative control lentivirus (control); TAM, 4-hydroxytamoxifen

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Breast Cancer Research

ISSN: 1465-542X

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