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Fig. 3 | Breast Cancer Research

Fig. 3

From: Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-integrin signaling pathway in tumor cells

Fig. 3

Tamoxifen induces β1-integrin expression through a mechanism that involves the EGFR and MAPK/ERK signaling pathways. (a) Levels of EGFR were detected by Western blotting using specific antibodies in cells. MCF-7R cells were treated with ethanol (control), TAM (1 μM) alone or with the addition of the specific signaling pathway inhibitor targeting to EGFR (AG1478; 6 μM; AG), MAPK/ERK (U0126; 10 μM) or PI3K (Wortmannin; 10 μM; WM). β1-integrin expression was detected by (b) Western blotting and (c) immunofluorescence analyses. Each experiment was repeated at least three times. Western blotting results are shown as fold-changes in optical density compared with the control, and normalized to β-actin. *P < 0.05. Ctrl, control; ITGB1, β1-integrin; TAM, 4-hydroxytamoxifen

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Breast Cancer Research

ISSN: 1465-542X

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