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Fig. 2 | Breast Cancer Research

Fig. 2

From: Acquisition of epithelial-mesenchymal transition phenotype in the tamoxifen-resistant breast cancer cell: a new role for G protein-coupled estrogen receptor in mediating tamoxifen resistance through cancer-associated fibroblast-derived fibronectin and β1-integrin signaling pathway in tumor cells

Fig. 2

Tamoxifen-induced β1-integrin expression is mediated by G protein-coupled estrogen receptor in MCF-7R cells. MCF-7 cells and MCF-7R cells were analyzed for the presence of β1-integrin by (a) RT-PCR, (b) Western blotting and (c) immunofluorescence. The relative fold-change was compared with MCF-7 cells (*P < 0.05, Student’s t-test). (d) β1-integrin was detected through Western blotting. MCF-7R cells were treated with ethanol (control), G1 (10 nM) and TAM (1 μM) alone or in combination with the G protein-coupled estrogen receptor (GPER) antagonist G15 (1 μM) for 24 hours. (e) Western blotting analysis was used to test the effect of GPER-specific siRNA on GPER (left panel) and β1-integrin (right panel) levels in MCF-7R cells. Each experiment was repeated at least three times. Results are shown as fold-changes in optical density compared with the control, and normalized to β-actin. *P < 0.05. Ctrl, control; ITGB1, β1-integrin; TAM, 4-hydroxytamoxifen

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Breast Cancer Research

ISSN: 1465-542X

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