Experimental workflow for magnetic hyperthermia in vivo. After tumor implantation, magnetic nanoparticles (MNP) were applied intratumorally 24 h prior to the first magnetic hyperthermia treatment (60 minutes, alternating magnetic field (AMF) H = 15.5 kA m−1, f = 435 kHz). Seven days later a second hyperthermia treatment was performed. Tumor volume, blood count and MNP distribution were monitored by micro computed tomography (μCT) on the indicated days. After the experimental period of 28 days the animals were sacrificied and the tumors dissected.