#hMADS-CM and hMADS-CM do not alter natural killer cell cytotoxicity. (A) Expression of markers and receptors on NK-92-CD16 cells from antibody-dependent cellular cytotoxicity (ADCC) assays in the presence of conditioned media of differentiated human multipotent adipose-derived stem cells (#hMADS-CM) or human multipotent adipose-derived stem cells (hMADS-CM) or in the control medium. Dotted red lines indicate unstained NK-92-CD16 cells, dotted green lines indicate control medium, solid coral lines indicate #hMADS-CM and dashed blue line indicate hMADS-CM conditions, respectively. (B) Enzyme-linked immunosorbent assay of the supernatants from NK-92-CD16 cells after ADCC assay. IFN, Interferon. (C) ADCC assays using NK-92-CD16 cells preincubated overnight with #hMADS-CM or hMADS-CM or the control media. (D) Cytotoxicity assay using K-562 cells as target cells at an effector to target ratio of 5:1 in the presence of #hMADS-CM, hMADS-CM or their control media. Results representative of three independent experiments are shown in (A). Mean ± SD values of three independent experiments, each performed in duplicate (B) or triplicate (C and D), are shown. *P < 0.05; **P < 0.01; ns, Not significant.