#hMADS adipocytes and hMADS preadipocytes inhibit antibody-dependent cellular cytotoxicity. (A) Schema of antibody-dependent cellular cytotoxicity (ADCC) assays in the absence (left) or the presence (right) of adipose cells. (B) ADCC assays on BT-474 and MDA-MB-453 cells as described in (A). (C) ADCC assay on BT-474 cells performed in hypoxic conditions with 1% O2. The cytotoxicity was normalized to the control differentiated medium as 100%. Mean ± SD values of three independent experiments, each performed in triplicate, are shown (B and C). hMADS, Human multipotent adipose-derived stem cells; #hMADS, Differentiated human multipotent adipose-derived stem cells; HME, Human mammary epithelial cell. *P < 0.05; **P < 0.01; ns, Not significant.