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Figure 5 | Breast Cancer Research

Figure 5

From: Granulocytic immune infiltrates are essential for the efficient formation of breast cancer liver metastases

Figure 5

Depletion of Gr-1 + (Ly-6C/Ly-6G) cells differentially affects the establishment and growth of bone, lung and liver metastases. (A) Schematic depicting the experimental protocol for depletion of Gr-1+ cells. (B) The degree of osteolytic bone destruction in the hindlimbs of mice treated with anti-Gr1 and isotype control antibodies was quantified by in vivo micro-computed. tomography (μCT) imaging. Bone volume of defined regions of the proximal tibia is shown following cardiac injection of 593 bone-aggressive cells. No difference was observed when comparing bone volumes between the two populations. Representative images of bone reconstructions are shown. (C) Quantification of the tumor burden (tumor area/tissue area) within the lung following tail vein injection of 526 lung-aggressive cells. No statistical difference in lung metastatic burden was observed when the isotype control cohort was compared with Gr-1+-depleted cohort. (D) Quantification of the tumor burden (tumor area/tissue area) within the cardiac liver lobe following splenic injection of 2776 liver-aggressive cells. Statistically significant decreases in liver-metastatic burden were observed when the isotype control cohort was compared with the Gr-1-depleted cohort (P = 0.012). Hematoxylin and eosin (H&E) images of the lung or cardiac liver lobe are shown for mice injected with each of the indicated cell populations and treated with isotype control or anti-Gr1 antibodies. Dotted lines circumscribe breast cancer metastatic lesions within the liver. Scale bar represents 2 mm and applies to all panels. IP: intraperitoneal injection; ns: not statistically significant.

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