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Figure 4 | Breast Cancer Research

Figure 4

From: Granulocytic immune infiltrates are essential for the efficient formation of breast cancer liver metastases

Figure 4

Ly-6G + cells are recruited in breast cancer-derived lung and liver metastases. Paraffin-embedded sections from primary breast tumors, bone metastases, lung metastases and liver metastases were obtained following experimental metastasis assays and subjected to immunohistochemical staining with anti-Ly-6G antibodies. (A) Representative images from 20X and 40X magnifications for each metastatic site are shown. 40X images were taken either at the margin of the lesions (40X margin) or in regions distal to the metastatic lesion (40X adj.). (B) Positivity of Ly-6G staining (expressed as a ratio of positive pixels over the total pixels per field) was quantified within the metastatic lesions (TUMOR), in close proximity to the metastatic lesion (PROX ADJ), in the tissue adjacent (ADJ) to the metastases or in control (CTRL) samples without any metastatic lesions. Increased recruitment of Ly-6G+ cells was observed in close proximity to hepatic metastases (*: liver tumor vs. liver adj., P <0.001; liver adj. vs. liver prox. Adj., P <0.001; lung tumor vs. lung adj., P <0.001). Scale bar represents 40 μm (20X) or 20 μm (40X) and applies to all panels of the same magnification.

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