Skip to main content

Table 1 Background information on the breast carcinoma cell lines used in this study

From: FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth

Cells

Source

Culture media

Cancer type

Selected genetic events [51]

MCF7

D. Cheresh (UCSD)

(1)

Invasive ductal carcinoma [52]

PIK3CA (E545K)

BT474

ATCC

(1)

Invasive ductal carcinoma [52]

PIK3CA (K111N)

TP53 (E285K)

HER2 (amplification)

MDA-MB-231

ATCC

(2)

Adenocarcinoma [52]

BRAF (G464V)

KRAS (G13D)

TP53 (R280K)

MDA-MB-468

ATCC

(2)

Adenocarcinoma [52]

PTEN (V85_splice)

4T1L

D Schlaepfer (UCSD)

(2)

Selection of metastatic mCherry-labeled 4 T1 cells by isolation and culture of cells from 4 T1 lung metastases [15]

(undetermined)

  1. (1) Roswell Park Memorial Institute medium supplemented with 10% FBS, 0.1 mM non-essential amino acids, 2 mM glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin. (2) DMEM supplemented with 10% FBS, 0.1 mM non-essential amino acids, 2 mM glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin.