Trafficking of c-Met in MDA-MB-468 cells. (A) In resting cells there is prominent membrane expression of the receptor (white arrowheads). (B) Following hepatocyte growth factor stimulation there is internalisation of the receptor and a predominantly perinuclear, granular pattern of staining (white arrowheads), consistent with the presence of c-Met within endosomes. Immunofluorescence (green, c-Met; blue, nuclei); ×63 objective under oil immersion. Scale bars represent 20 μm.