Mouse mammary stem cells express prognostic markers for triple-negative breast cancer

Breast Cancer Research

Table 1 Results of staining of cells sorted on slides for keratins 14 (K14) and 18 (K18) and ERα

Population	Antigen (number of cells counted)	Staining (%)
Population	Antigen (number of cells counted)	-	−/+	+
MaSCs	K14 (106)	0	19	81
	K18 (106)	98	2	0
	ERα (62)	100		0
MYOs	K14 (79)	16	48	35
	K18 (79)	100	0	0
	ERα (60)	100		0
Luminal ER-	K14 (128)	100	0	0
	K18 (128)	2	52	46
	ERα (65)	96		4
Luminal ER+	K14 (118)	100	0	0
	K18 (118	0	20	80
	ERα (60)	0		100

Cells were either double-stained for K14/K18 or single-stained for ERα and then counterstained with DAPI. Examples of negative (−), weak (−/+) and positive (+) staining and of double-stained MaSCs are shown in Additional file 5. ERα staining was only scored as negative or positive. ERα, estrogen receptor alpha; MaSC, mammary stem cell; MYO, myoepithelial cell.

ISSN: 1465-542X