Figure 6
PCI and Tam combination alters expression of pro-apoptotic proteins and promotes apoptosis. (A) Tamoxifen-resistant cells derived from MCF7 (TAMRM) cells were treated with increasing doses of PCI-24781 (PCI) for 72 hours and expression of indicated proteins were evaluated by western blot. (B) MCF7 and TAMRM cells were treated with vehicle (C), 200 nM PCI (P), 10 μM 4-hydroxy tamoxifen (Tam) (T) or the combination of PCI-24781 and 4-hydroxy tamoxifen (PT) for 72 hours and PARP cleavage and expression of pro-apoptotic proteins was evaluated by western blot.