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Figure 6 | Breast Cancer Research

Figure 6

From: FOXF2 deficiency promotes epithelial-mesenchymal transition and metastasis of basal-like breast cancer

Figure 6

FOXF2 deficiency induces EMT of basal-like mammary epithelial cells via upregulation TWIST1 transcription. (A) The expression of TWIST1 and EMT markers in the indicated cells was detected by immunoblot. (B) Morphology of the indicated cells (×200). (C) Photos (Lift) and counts (Right) of migration and invasion of the indicated cells assessed by transwell assay (×200). Three independent experiments were performed in triplicate. The data were expressed as mean ± SD. ** P <0.01. (D) The expression of TWIST1 and vimentin in the indicated cells was detected by immunoblot. (E) The migration and invasion ability of the indicated cells were assessed by transwell assay. Three independent experiments were performed in triplicate. The data were expressed as mean ± SD. ** P <0.01. (F) The TWIST1 protein expression in primary tumor tissues harvested from xenograft mice of MDA-MB-231-Luc shFOXF2 and MDA-MB-231-Luc shControl cells was stained by immunohistochemistry (×400). (G) The correlation of FOXF2 mRNA levels with TWIST1 mRNA levels in human primary TNBC tissues (n = 34; Spearman’s correlation rho = −0.421, P = 0.013). (H) The correlation of combined FOXF2 and TWIST1 mRNA levels with the DFS of TNBC patients with FOXF2 high/TWIST1 low (n = 8), FOXF2 high/TWIST1 high (n = 8), FOXF2 low/TWIST low (n = 8) and FOXF2 low/TWIST1 high (n = 10) was estimated by a Kaplan-Meier survival analysis. DFS, disease-free survival; EMT, epithelial-mesenchymal transition; SD, standard deviation; TNBC, triple-negative breast cancer.

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