Figure 1

FOXF2 deficiency enhances migration and invasion of basal-like mammary epithelial cells and breast cancer cells in vitro. (A) FOXF2 mRNA levels in the indicated cell lines were measured by RT-qPCR. The data were normalized to the expression of the housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH). (B) The protein expression of FOXF2 in the indicated cells was detected by immunoblot. (C) FOXF2 mRNA levels in different subtypes of breast cell lines were analyzed using an independent GOBO dataset. (D) FOXF2 mRNA levels in the indicated cells were measured by RT-qPCR. Fold changes were relative to the mRNA expression of the control cells. (E) The migration and invasion ability of the indicated cells were assessed by transwell assay. (F) Proliferation of the indicated cells was determined by MTT assay. Three independent assays were performed in triplicate. The data were expressed as mean ± SD. ^** P <0.01. GOBO, Gene expression-based Outcome for Breast cancer Online; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; RT-qPCR, reverse transcription quantitative PCR; SD, standard deviation.