Table 1 Collected RNA and RNA integrity number values of laser-microdissected breast tumor cells using Bioanalyzer and Nanodrop analysis
Extracted RNA | |||||
|---|---|---|---|---|---|
Isolated tissue area | Bioanalyzer | Nanodrop | |||
mm 2 | ng/μL | RIN | ng/μL | 260/280 | |
A) | |||||
Malignant epithelial cells | 28 | 16.8 | 2.6 | 44.8 | 2.1 |
Benign epithelial cells | 25 | 11.1 | 2.7 | 24.7 | 2.24 |
Stroma cells (including inflammatory cells) | 18.2 | 10.2 | 2.3 | 16.2 | 2.26 |
Stroma cells | 18.4 | 0.4 | N/A | 2.5 | −26 |
Total tissue section | ~200 | 200 | 2.3 | 282 | 2.07 |
B) | |||||
Epithelial cells | 32.65 | 57.0 | 2.8 | 23.31 | 2.06 |
Stroma cells | 26.5 | 27.0 | 2.4 | 15.59 | 1.97 |
Total tissue section | ~200 | 266 | 3.0 | 230.5 | 2.05 |
C) | |||||
Epithelial cells | 19.25 | 27.7 | 2.6 | 30.62 | 2.06 |
Stroma cells | 92.35 | 10.5 | 2.1 | 25.33 | 1.89 |
Total tissue section | ~200 | 28.8 | 2.3 | 48.53 | 1.59 |
D) | |||||
Epithelial cells | 10.2 | 1.3 | 1.2 | 10.14 | 1.94 |
Stroma cells | 15.0 | 7.8 | 2.1 | 21.77 | 1.63 |
Total tissue section | ~200 | 5.6 | 2.2 | 14.51 | 1.54 |