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Figure 3 | Breast Cancer Research

Figure 3

From: In silico discovery and validation of potent small-molecule inhibitors targeting the activation function 2 site of human oestrogen receptor α

Figure 3

The dose–response curves of the lead compounds are right-shifted in the presence of higher concentrations of coactivator peptide. Dose–response curves (0.05 to 400 μM) of (A) VPC-13002, (B) VPC-16225 and (C) VPC-16230 showing a rightward shift with increases in concentrations of fluorescein- peroxisome proliferator-activated receptor gamma, coactivator 1-alpha (PGC-1α) generated by the time-resolved fluorescence resonance energy transfer assay. Error bars indicate standard error of mean for n = 6 values. Data were fitted using GraphPad Prism 5 to calculate the log of the concentration of the inhibitors versus emission ratio.

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