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Table 3 The most prominent microRNA detection methods

From: Dysregulation of microRNAs in breast cancer and their potential role as prognostic and predictive biomarkers in patient management

Detection method Throughput Sensitivity Specificity Reference
Northern blotting Low Low High [53]
Microarray High Low Low [54]
Bead-based flow cytometry High Medium High [6]
qRT-PCR High High High [55]
In situ hybridization Low Low Low [56]
Next-generation sequencing High High High [57,58]
  1. qRT-PCR, quantitative real-time polymerase chain reaction.