Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy

Kren, Betsy T; Unger, Gretchen M; Abedin, Md J; Vogel, Rachel I; Henzler, Christine M; Ahmed, Khalil; Trembley, Janeen H

doi:10.1186/s13058-015-0524-0

Table 4 Protein expression levels following small interfering RNA transfection

From: Preclinical evaluation of cyclin dependent kinase 11 and casein kinase 2 survival kinases as RNA interference targets for triple negative breast cancer therapy

	siRNA	CDK11	Cyclin L1α	Cyclin L2α	CK2α	CK2α ′	CK2β	Caspase 3 – full length	Bcl-xL	Survivin
MDA-MB-231	siCDK11/CK2	0.09 ± 0.02	3.07 ± 1.56	0.73 ± 0.53	0.07 ± 0.08	0.05 ± 0.06	0.03 ± 0.03	0.45 ± 0.17	0.40 ± 0.38	0.73 ± 0.53
	siCDK11/CK2	P <0.0001			P <0.0001	P = 0.001	P <0.0001	0.45 ± 0.17	0.40 ± 0.38	0.73 ± 0.53
	siCK2	0.98 ± 0.17	1.82 ± 0.67	0.78 ± 0.45	0.07 ± 0.07	0.05 ± 0.06	0.06 ± 0.04	0.85 ± 0.57	0.62 ± 0.10	0.85 ± 0.33
	siCK2	0.98 ± 0.17	1.82 ± 0.67	0.78 ± 0.45	P <0.0001	P = 0.001	P <0.0001	0.85 ± 0.57	0.62 ± 0.10	0.85 ± 0.33
	siCDK11	0.04 ± 0.07	2.38 ± 0.18	0.91 ± 0.49	0.88 ± 0.17	1.13 ± 0.18	1.05 ± 0.15	0.57 ± 0.23	0.24 ± 0.05	0.79 ± 0.21
	siCDK11	P <0.0001	2.38 ± 0.18	0.91 ± 0.49	0.88 ± 0.17	1.13 ± 0.18	1.05 ± 0.15	0.57 ± 0.23	P = 0.022	0.79 ± 0.21
	siControl	0.90 ± 0.04	1.77 ± 0.31	1.44 ± 0.62	1.09 ± 0.10	0.98 ± 0.26	1.14 ± 0.04	0.99 ± 0.07	0.93 ± 0.32	1.02 ± 0.20
SUM-149	siCDK11/CK2	0.44 ± 0.04	1.05 ± 0.12	1.30 ± 0.48	0.06 ± 0.05	0.27 ± 0.10	0.25 ± 0.14	0.45 ± 0.17	0.31 ± 0.21	0.77 ± 0.33
	siCDK11/CK2	P <0.0001	1.05 ± 0.12	1.30 ± 0.48	P <0.0001	P = 0.0004	P = 0.001	P = 0.036	P = 0.034	0.77 ± 0.33
	siCK2	0.86 ± 0.13	0.93 ± 0.18	1.31 ± 0.52	0.08 ± 0.07	0.31 ± 0.23	0.14 ± 0.07	0.55 ± 0.34	0.73 ± 0.12	1.02 ± 0.10
	siCK2	0.86 ± 0.13	0.93 ± 0.18	1.31 ± 0.52	P <0.0001	P = 0.0007	P = 0.0004	0.55 ± 0.34	0.73 ± 0.12	1.02 ± 0.10
	siCDK11	0.40 ± 0.16	1.04 ± 0.05	1.00 ± 0.19	1.19 ± 0.14	0.96 ± 0.12	0.77 ± 0.26	0.62 ± 0.23	0.54 ± 0.31	0.98 ± 0.18
	siCDK11	P <0.0001	1.04 ± 0.05	1.00 ± 0.19	1.19 ± 0.14	0.96 ± 0.12	0.77 ± 0.26	0.62 ± 0.23	0.54 ± 0.31	0.98 ± 0.18
	siControl	0.89 ± 0.12	0.89 ± 0.26	0.90 ± 0.87	1.36 ± 0.27	1.05 ± 0.29	0.88 ± 0.17	0.92 ± 0.24	1.08 ± 0.30	0.89 ± 0.22

All values normalized to actin expression and expressed relative to untreated cells as mean ± standard deviation. P values are listed underneath the corresponding protein expression value for comparison with untreated cells if P <0.05. CDK, cyclin-dependent kinase; CK2, casein kinase 2; siRNA, small interfering RNA.

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