| |
siRNA
|
CDK11
|
Cyclin L1α
|
Cyclin L2α
|
CK2α
|
CK2α
′
|
CK2β
|
Caspase 3 – full length
|
Bcl-xL
|
Survivin
|
|---|
|
MDA-MB-231
|
siCDK11/CK2
|
0.09 ± 0.02
|
3.07 ± 1.56
|
0.73 ± 0.53
|
0.07 ± 0.08
|
0.05 ± 0.06
|
0.03 ± 0.03
|
0.45 ± 0.17
|
0.40 ± 0.38
|
0.73 ± 0.53
|
| |
P <0.0001
| | |
P <0.0001
|
P = 0.001
|
P <0.0001
|
| |
siCK2
|
0.98 ± 0.17
|
1.82 ± 0.67
|
0.78 ± 0.45
|
0.07 ± 0.07
|
0.05 ± 0.06
|
0.06 ± 0.04
|
0.85 ± 0.57
|
0.62 ± 0.10
|
0.85 ± 0.33
|
| |
P <0.0001
|
P = 0.001
|
P <0.0001
|
| |
siCDK11
|
0.04 ± 0.07
|
2.38 ± 0.18
|
0.91 ± 0.49
|
0.88 ± 0.17
|
1.13 ± 0.18
|
1.05 ± 0.15
|
0.57 ± 0.23
|
0.24 ± 0.05
|
0.79 ± 0.21
|
| |
P <0.0001
|
P = 0.022
|
| |
siControl
|
0.90 ± 0.04
|
1.77 ± 0.31
|
1.44 ± 0.62
|
1.09 ± 0.10
|
0.98 ± 0.26
|
1.14 ± 0.04
|
0.99 ± 0.07
|
0.93 ± 0.32
|
1.02 ± 0.20
|
|
SUM-149
|
siCDK11/CK2
|
0.44 ± 0.04
|
1.05 ± 0.12
|
1.30 ± 0.48
|
0.06 ± 0.05
|
0.27 ± 0.10
|
0.25 ± 0.14
|
0.45 ± 0.17
|
0.31 ± 0.21
|
0.77 ± 0.33
|
| |
P <0.0001
|
P <0.0001
|
P = 0.0004
|
P = 0.001
|
P = 0.036
|
P = 0.034
|
| |
siCK2
|
0.86 ± 0.13
|
0.93 ± 0.18
|
1.31 ± 0.52
|
0.08 ± 0.07
|
0.31 ± 0.23
|
0.14 ± 0.07
|
0.55 ± 0.34
|
0.73 ± 0.12
|
1.02 ± 0.10
|
| |
P <0.0001
|
P = 0.0007
|
P = 0.0004
|
| |
siCDK11
|
0.40 ± 0.16
|
1.04 ± 0.05
|
1.00 ± 0.19
|
1.19 ± 0.14
|
0.96 ± 0.12
|
0.77 ± 0.26
|
0.62 ± 0.23
|
0.54 ± 0.31
|
0.98 ± 0.18
|
| |
P <0.0001
|
| |
siControl
|
0.89 ± 0.12
|
0.89 ± 0.26
|
0.90 ± 0.87
|
1.36 ± 0.27
|
1.05 ± 0.29
|
0.88 ± 0.17
|
0.92 ± 0.24
|
1.08 ± 0.30
|
0.89 ± 0.22
|
- All values normalized to actin expression and expressed relative to untreated cells as mean ± standard deviation. P values are listed underneath the corresponding protein expression value for comparison with untreated cells if P <0.05. CDK, cyclin-dependent kinase; CK2, casein kinase 2; siRNA, small interfering RNA.
