Figure 1
From: Patient-derived xenograft models of breast cancer and their predictive power
Derivation of patient-derived xenograft models of human breast cancer in mice. Tumor slices are implanted into the cleared inguinal fat pad of mice (MFP) concurrently with a subcutaneous estradiol pellet. Following growth of the tumor in passage 1 (T1), a single-cell suspension or tumor fragments can be prepared for sequential passage of the tumor. It is advisable to prepare frozen aliquots of cell suspensions or tumor fragments at T1 and T2, as a source of early passage tumor.