Skip to main content

Advertisement

Table 1 Relative effects of the 47 target genes in two-dimensional and three-dimensional cultures a

From: A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation

Gene name RefSeq ID Relative rank, primary screen ( N= 47) Median relative rank, secondary screen ( N= 47) Relative rank, three-dimensional growth assay ( N= 44) Three-dimensional acinar formation, fold decrease (siRNA) shRNA knockdown effect on clone
ACE2 NM_021804 22 30 11 5.169 Lethal
ADCY4 NM_139427 44 17 44 0.878 Lethal
BDKRB2 NM_000623 28 44 31 2.484 Growth postselection
BST1 NM_004334 7 6 14 4.406 Lethal
CD79A NM_001783 13 16 33 2.366 Growth postselection
COL9A3 NM_001853 16 4 22 3.489 Lethal
CTNNA1 NM_001903 37 29 18 4.047 Lethal
EFNAA NM_005227 38 37 5 7.338 Growth postselection
FIT1 NM_203402 4 22 No data No data Growth postselection
FL_J30634 NM_153014 15 30 17 4.055 Growth postselection
FLOT2 NM_004475 23 31 41 1.618 Lethal
FZD2 NM_001466 6 25 19 3.7126 Lethal
GPR182 NM_007264 9 20 32 2.483 Growth postselection
GPR39 NM_001508 10 22 26 3.159 Growth postselection
GPR80 NM_080818 32 14 27 3.137 Lethal
HSD17B2 NM_002153 27 38 2 27.328 Lethal
KCNJ5 NM_000890 3 35 42 1.494 Growth postselection
KTELC1 NM_020231 8 17 No data No data Growth postselection
LGALS1 NM_002305 36 7 16 4.120 Growth postselection
LPAR3 NM_012152 1 5 20 3.530 Growth postselection
LTBP3 NM_021070 39 28 9 5.291 Growth postselection
MMP24 NM_00690 40 5 8 5.447 Growth postselection
MMP28 NM_024302 18 25 37 2.075 Lethal
NKAIN4 NM_152864 20 9 21 3.505 Lethal
NPTX1 NM_002522 45 18 6 7.082 Lethal
NTN1 NM_004822 47 22 10 5.175 Growth postselection
NTN2L NM_006181 5 30 13 4.691 Growth postselection
OPRS1 NM_005866 29 26 24 3.243 Lethal
PARD3 NM_019619 30 31 15 4.221 Lethal
PCDHB13 NM_018933 46 27 43 1.214 Growth postselection
PDCD1 NM_00518 21 36 28 2.997 Growth postselection
PLA2G2F NM_022819 19 22 38 2.028 Lethal
PLUNC NM_016583 31 30 23 3.280 Growth postselection
PROCR NM_006404 35 36 1 31.637 Growth postselection
RHCE NM_020485 42 31 35 2.209 Lethal
RIPK2 NM_003821 41 23 No data No data Lethal
ROBO3 NM_022370 11 35 39 2.012 Growth postselection
SAA1 NM_000331 14 21 12 5.167 Lethal
SCARB2 NM_005506 34 9 34 2.317 Lethal
SEMA3C NM_006379 12 25 40 1.936 Growth postselection
SERPINH1 NM_001235 26 32 25 3.187 Lethal
SLC6A4 NM_001045 17 29 36 2.142 Growth postselection
SNN NM_003498 24 30 3 11.523 Lethal
TMEM14C NM_016462 33 32 7 5.884 Lethal
TMEM9B NM_020644 43 23 30 2.556 Lethal
TUFT1 NM_020127 25 20 4 7.399 Lethal
   /47 /47 /44   
  1. aThe relative rank of target genes in the primary small interfering RNA (siRNA) screen, the median relative rank in the secondary siRNA screen and the relative rank in the three-dimensional acinar growth assay are displayed. The fold decrease in structure formation for each target gene is displayed for each siRNA relative to the control condition. The effect of infection with an independent lentiviral short-hairpin (shRNA) clone against each target gene is shown after selection with puromycin (resistance marker within lentiviral construct) as a binary effect (lethal or growth postselection).