Figure 7

LPAR3 , but not LPAR1 or LPAR2 , is necessary for two-dimensional and three-dimensional mammary growth. Equal numbers of 184-hTERT-L9 cells transfected with small interfering RNA (siRNA) pools against three lysophosphatidic acid (LPA) receptors (LPAR1, LPAR2 and LPAR3), transfection reagent alone and PLK1 (as a positive control) were plated in two-dimensional colony-forming assays (n = 3) (A) and three-dimensional Matrigel cultures (n = 4) (B). Cells were counted after 8 days in culture. Error bars represent 95% confidence intervals.