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Figure 6 | Breast Cancer Research

Figure 6

From: A co-culture genome-wide RNAi screen with mammary epithelial cells reveals transmembrane signals required for growth and differentiation

Figure 6

PROCR expression is necessary for cell organization and luminal clearance in three-dimensional culture. 184-hTERT-L9 cell lines with stable integration of pGIPZ short-hairpin RNA (shRNA) lentiviral constructs against PROCR or a nontargeting control were seeded into three-dimensional Matrigel culture and fixed after 21 days of growth. Staining was performed with Alexa Fluor 546–conjugated phalloidin and DRAQ5 nuclear stain prior to imaging on a Nikon confocal laser scanning microscope. GFP, Green fluorescent protein.

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