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Figure 2 | Breast Cancer Research

Figure 2

From: Cancer progression by breast tumors with Pit-1-overexpression is blocked by inhibition of metalloproteinase (MMP)-13

Figure 2

Pit-1 binds to the MMP-1 and MMP-13 promoter. (A) Diagram of the human MMP-1 gene promoter showing the putative Pit-1 binding sites, and the location of the primers used in the ChIP assay. (B) Soluble chromatin prepared from control, pRc/RSV, or pRSV-hPit-1-transfected MCF-7 cells were immunoprecipitated with an anti-Pit-1 antibody or control IgG. The immunoprecipitated DNA was amplified by PCR using primers (A, B, C, and D) that amplified regions of the MMP-1 promoter with or without the putative Pit-1 binding sites. (C) Diagram of the human MMP-13 gene promoter showing the putative Pit-1 binding sites, and the location of the primers used in the ChIP assay. (D) Soluble chromatin and immunoprecipitation were performed as described in B. The immunoprecipitated DNA was amplified by PCR using primers (A, B, C, D, and E) that amplified regions of the MMP-13 promoter. ChIP, chromatin immunoprecipitation; MMP-1, matrix metalloproteinase-1; MMP-13, matrix metalloproteinase-13; Pit-1, POU class 1 homeobox 1.

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