Silencing of p53 and activation of PI3K in 4G cells. A. Western blots showing that the shp53 vector silences p53 expression, and the PIK3CA vector increases PI3K expression and AKT phosphorylation on T308. B. Growth curves comparing parental 4G cells with cells infected with the shp53 and PIK3CA vectors. Error bars sem; † p < 0.001; * p < 0.05; n = 5 biological replicates.