TY - JOUR AU - Verbeke, Stephanie AU - Richard, Elodie AU - Monceau, Elodie AU - Schmidt, Xenia AU - Rousseau, Benoit AU - Velasco, Valerie AU - Bernard, David AU - Bonnefoi, Herve AU - MacGrogan, Gaetan AU - Iggo, Richard D. PY - 2014 DA - 2014/12/20 TI - Humanization of the mouse mammary gland by replacement of the luminal layer with genetically-engineered preneoplastic human cells JO - Breast Cancer Research SP - 504 VL - 16 IS - 6 AB - The cell of origin for estrogen receptor α (ERα) positive breast cancer is probably a luminal stem cell in the terminal duct lobular units. To model these cells we have used the murine myoepithelial layer in the mouse mammary ducts as a scaffold upon which to build a human luminal layer. To prevent squamous metaplasia, a common artifact in genetically engineered breast cancer models, we sought to limit activation of the epidermal growth factor receptor (EGFR) during in vitro cell culture before grafting the cells. SN - 1465-542X UR - https://doi.org/10.1186/s13058-014-0504-9 DO - 10.1186/s13058-014-0504-9 ID - Verbeke2014 ER -