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Figure 5 | Breast Cancer Research

Figure 5

From: Toca-1 is suppressed by p53 to limit breast cancer cell invasion and tumor metastasis

Figure 5

p53 silencing enhances MTLn3 tumor progression and lung metastasis and partial rescue with Toca-1 silencing. Mammary orthotopic xenograft assays using MTLn3 control, sh-p53 and sh-p53/sh-Toca-1 cells were analyzed at 5 weeks post injection of Rag2-/-IL2Rγc-/- mice. (A) Graph depicts primary tumor mass for each mouse injected with the indicated cell line (mean ± SD; n = 5 mice/group; significant differences between groups based on paired Student’s t test are indicated by *P <0.05 or ***P <0.001). (B) Levels of Toca-1 and p53 in the tumor homogenates were analyzed by IB (β-actin served as a loading control; dashed lines indicating removal of additional lanes). (C) Representative H&E-stained sections of lungs from mice harboring mammary tumors for the indicated cell lines are shown (arrows depict metastatic nodules). (D) Graph depicts the number of metastases per lung for each mouse injected with the indicated cell line (mean ± SD; n = 5 mice/group; significant differences between groups based on paired Student’s t test are indicated by *P <0.05 or ***P <0.001). H&E, hematoxylin and eosin; IB, immunoblot; SD, standard deviation; Toca-1, transducer of Cdc42-mediated actin assembly.

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