Figure 1

Toca-1 expression correlates with p53 status in breast cancer cell lines. (A) Lysates from the indicated normal-like breast epithelial and breast cancer cell lines were grouped according to either WT or mutant p53 and subjected to IB with Toca-1, p53 and β-Actin antibodies. (B) Densitometric analysis of Toca-1 protein levels relative to β-actin in WT p53 and mutant p53 cell lines (mean ± SD); ^*depicts a significant difference based on paired Student’s t test (P <0.05)). (C) p53-null HCC1806 cells were transfected with mutant p53 (Ala-143, Trp-248) plasmids and the levels of Toca-1 and p53 were assessed by IB (actin served as a loading control). (D) siRNA mediated silencing of endogenous mutant p53 in HCC-38 and SKBR3 cell lines. Levels of Toca-1 and p53 were assessed by IB (actin served as a loading control). IB, immunoblot; siRNA, small interfering RNA; SD, standard deviation; Toca-1, transducer of Cdc42-mediated actin assembly; WT, wild-type.