Mammary ductal branching density in post-coitus mice. (A) High-resolution composite digital light microscopic representative images of 12-days post-coitus (12d PC) and 1 day post-partum (1d PP) control, CYT-1, and CYT-2 mice were recorded using an Axio Imager.A.1 microscope with an AxioCam MRc5 camera and AxioVision 4.7 imaging software (Zeiss, Thornwood, NJ, USA), or (B) a SPOT 11.2 Color Mosaic camera (Diagnostic Instruments Inc; Sterling Heights, MI, USA) for a magnified image. (C) 12d PC whole mount mammary glands were blinded and scored over a range of 1 to 10 for highest to lowest branching density after microscopic inspection. Data points indicate percent growth ± standard deviation in each group *P <0.05 by unpaired t test. (D) Representative hematoxylin and eosin (HE) image of control and CYT-1 PC-12 mammary gland thin section. Scale bars = 50 μm.