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Figure 7 | Breast Cancer Research

Figure 7

From: Bone marrow stromal antigen 2 expressed in cancer cells promotes mammary tumor growth and metastasis

Figure 7

Endogenous BST-2 expressed in mammary cancer cells controls cell migration and invasion. (A) Representative images of cell migration performed by scratch assay. Suppression of BST-2 expression by sh137 and sh413 reduced rate of cancer cell migration into the scratch wound. (B) Quantification of cells that migrated into the scratch wound at 0, 6, and 24 h. (C) Migration assay by Boyden chamber. Representative images taken at 4X. (D) Migrated cells were imaged and the number of migrated cells counted with Image J. Similar results were obtained with 4T1 shControl and sh413 cells. (E) BST-2-expressing shControl and BST-2 suppressed sh137 and sh413 E0771 cells and (F) shControl and sh413 4T1 cells were plated in low-serum medium on Matrigel-coated cell inserts and allowed to migrate for 24 h. Cells were stained with Giemsa stain. Representative images taken at 20X are shown. (G) Quantification of cells that invaded through Matrigel. Cells from five different fields were counted and averaged. Error bars corresponds to standard deviations. Significance was taken at P <0.001** and P <0.05*. Experiments were repeated multiple times with similar results.

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