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Figure 6 | Breast Cancer Research

Figure 6

From: Embryonic cells contribute directly to the quiescent stem cell population in the adult mouse mammary gland

Figure 6

eLLRCs from the MaSC population generate multi-lineage mammospheres. (A) Three-dimensional image of mammosphere stained for EdU (red), K14 (white) and K18 (green). Representative bright field images of gross morphology of primary (B) and secondary (D) mammospheres grown under ultra low non-adherent conditions in vitro. (C , E) Immunofluorescence analysis of paraffin sections of mammospheres stained for EdU (red) with nuclear counterstain, DAPI (blue). C represents three different primary mammospheres while E is a second-generation mammosphere. DAPI, 4′,6-diamidino-2-phenylindole; EdU, 5-ethynl-2′-deoxyuridine; eLLRCs, embryonically derived long label retaining cells; MaSC, mammary stem cell.

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