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Figure 3 | Breast Cancer Research

Figure 3

From: Embryonic cells contribute directly to the quiescent stem cell population in the adult mouse mammary gland

Figure 3

Embryonic EdU labeling followed by pubertal BrdU labeling demonstrates overlapping but distinct LLRC populations. (A) Scheme for sequential embryonic labeling with EdU from e14.5 to e18.5 followed by pubertal labeling with BrdU from day 21 to 25. (B) Diagrammatic representation of the relative localization of EdU-retaining and BrdU-retaining epithelial cells in the adult mammary gland at eight-weeks of age. EdU-retaining cells localized to the main ducts in the nipple region, while BrdU cells are localized between the EdU-positive cells and the lymph node. (C) Percentage of EdU-positive and BrdU-positive cells at different time points following labeling. Representative sections co-stained for BrdU and the myoepithelial markers K14 (D) or p63 (E), or the luminal markers, estrogen receptor (F) or progesterone receptor (G). Boxes represent areas of inset. Arrows in insets point to double-labeled cells. (H) Percentage of BrdU-positive cells that also stain for K14, p63, ER or PR. BrdU, 5-bromo-2′-deoxyuridine; e, embryonic day; EdU, 5-ethynl-2′-deoxyuridine; ER, estrogen receptor; LLRC, long label retaining cells.

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