Figure 2From: Evaluation of proliferation and apoptosis markers in circulating tumor cells of women with early breast cancer who are candidates for tumor dormancyExpression of the proliferation marker Ki67 and the apoptotic marker M30 in CTCs of patients with early breast cancer. (A) Representative image of a CTC stained positive for the proliferation marker Ki67 along with PBMCs. (B) Representative image of a CTC stained positive for the apoptotic marker M30. Cytospins were triple stained with pancytokeratin rabbit antibody/secondary anti-rabbit Alexa Fluor 555 (orange), anti-Ki67 mouse antibody/secondary anti-mouse Alexa 633 (red) and M30 mouse FITC-conjugated antibody (green). Cell nuclei were stained with DAPI (blue). Images were taken by ARIOL system (X60). ARIOL system, automated image analysis system; CTCs, circulating tumor cells; DAPI, 4’,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate; PBMCs, peripheral blood mononuclear cells.Back to article page