Expression of cytokeratin and M30 or cytokeratin and Ki67 in SKBR3 and MDA-MB231 breast cancer cell lines respectively. (A) Staurosporine-treated SKBR3 cells were triple stained with pancytokeratin rabbit antibody/secondary anti-rabbit Alexa Fluor 555 (orange), anti-Ki67 mouse antibody/secondary anti-mouse Alexa 633 (red) and M30 mouse FITC-conjugated antibody (green). Cell nuclei were stained with DAPI (blue). Images were obtained by the use of ARIOL system (X60). (B) MDA-MB231 cells were triple stained as described above. The positive nuclear dotted staining (red) was evaluated for Ki67 staining. Images were obtained by the use of ARIOL system (X60). ARIOL system, automated image analysis system; DAPI, 4’,6-diamidino-2-phenylindole; FITC, fluorescein isothiocyanate.