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Figure 3 | Breast Cancer Research

Figure 3

From: A novel phosphatidylinositol 3-kinase (PI3K) inhibitor directs a potent FOXO-dependent, p53-independent cell cycle arrest phenotype characterized by the differential induction of a subset of FOXO-regulated genes

Figure 3

Transcription factor analysis. (A) Graph of transcription factors enriched in significantly upregulated genes following ETP-45658 treatment. The white bars indicate the motifs analysed in 3B). (B) Analysis of conserved sequence motifs for FOXO transcription factors (white bars in 3A) showing the enrichment of FOXO-binding sites within our genome-wide screen. The TRANSFAC matrix table entries for the indicated FOXO-binding sites are M00472, M00477, M00474 and M00473 respectively. (C) Representative immunoblots showing the effectiveness of FOXO3a or p53 knockdown in MCF-7 or U2OS cell lines following selection. (D) Cell cycle profile 48 hours post 100 nM ETP-45658 treatment in MCF-7- or U2OS-treated cells, N = 4. (E) Immunoblot showing little to no p53 accumulation following ETP-45658 exposure. (F) FACS analysis following 100 nM ETP-45658 treatment in MDA-MB231, Saos2 or (p53 −/−) HCT-116 cells N = 4.

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Breast Cancer Research

ISSN: 1465-542X

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