Hormone metabolism pathway genes and mammographic density change after quitting estrogen and progestin combined hormone therapy in the California Teachers Study

Table 4 SNPs that are statistically significantly associated with EPT-associated mammographic density change in either nulliparous (n = 63) or parous (n = 219) women after correcting for multiple testing at gene level ^¶

SNP		Minor	Nulliparous	(n = 63)			Parous	(n = 219)
(major/minor allele)	Gene	allele frequency	N (WW/WV/VV)	Beta (SE)	P	P _ACT ^*	N (WW/WV/VV)	Beta (SE)	P	P _ACT ^*	P for interaction ^†
rs2077647 (T/C)	ESR1	0.49	18/30/15	3.92 (1.06)	0.0005	0.006	56/118/43	−0.40 (0.63)	0.53	>0.99	0.003
rs9605030 (C/T)	COMT	0.14	40/21/2	4.47 (1.42)	0.0025	0.043	166/48/4	0.69 (0.88)	0.44	>0.99	0.011
rs5933863 (G/A)	ARSC	0.15	46/17/0	1.26 (1.88)	0.51	0.75	152/62/5	2.00 (0.81)	0.014	0.027	0.82

^¶Based on linear regression model adjusting for age and BMI (kg/m²) at time of on-EPT mammogram, time interval and BMI change between the two mammograms, and mammographic density of on-EPT mammogram. Additive genetic model was used. *Multiple testing corrected P value; P _ACT (P values adjusted for correlated tests) within each gene was calculated using the methods by Conneely and Boehnke [33]. ^†P values for interaction were not corrected for multiple testing. SNP, single nucleotide polymorphisms; EPT, estrogen and progestin combined therapy; SE, standard error; BMI, body mass index.

ISSN: 1465-542X