Figure 6

Inhibition of miR-183/-96/-182 cluster miRNAs decreased cell proliferation, and even induced cell death. (A) Real-time PCR results showed the knockdown efficiency and specificity of miR-Down™ antagomirs: left panel, MCF-7 cells; right panel, T47D cells. U6 snRNA was used as internal control. Error bars represent SD (n = 4). (B) The 3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide (MTT) assays showed the cell growth rates of miR-183/-96/-182 cluster knockdown cells. Error bars represent SD (n = 4). (C) Cell wound-healing assays showed the migration abilities of miR-183/-96/-182 cluster knockdown cells. Error bars represent SD (n = 4). (D) Flow cytometric analysis of miR-183/-96/-182 cluster knockdown cells. Error bars represent SD (n = 3). (E) T47D cells infected with miR-183/-96/-182 cluster sponge lentivirus underwent apoptosis 3 days after transduction: left panels, phase-contrast micrographs of indicated cells; right panels, green fluorescent micrographs of indicated cells. Scale bars: 20 μM. (F) Analysis of miR-183/-96/-182 cluster sponge lentivirus-infected T47D cells: upper panel, inhibition efficiency of miR-183/-96/-182 cluster sponge lentivirus shown by real-time PCR (error bars represent SD, n = 4; lower panels, flow cytometric graph of indicated cells.