MiR-183/-96/-182 cluster is highly expressed in breast cancer cells. (A) Detection of miR-183/-96/-182 cluster miRNAs by LNA-based northern blot in different cancer samples and their normal adjacent tissues (NAT). Left panel shows the northern blot analysis of miR-183/-96/-182 cluster miRNAs; 5S-r RNA was used as an internal control. Left panel is the quantification of selected miRNAs by the Image J program. The results were normalized to the 5S-r RNA. (B) Statistical analysis of miRNA expression data in breast invasive carcinoma from the TCGA dataset: upper panel compares the miRNA expression levels between tumor samples and their matched normal samples; lower panel analyzes the correlation between miR-182 and miR-183 levels in normal and tumor samples. Error bars indicate SD (n = 102). (C) Quantification of the miR-183/-96/-182 cluster miRNAs by real-time PCR in different breast cancer cell lines. MCF-10A cell was used as control. U6 snRNA was used as internal control. Error bars indicate SD (n = 3).