Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome-wide promoter microarrays

Table 2 RU and RR cell populations are detectable in primary patient breast tumors

Patient number	Infection efficiency (RFP+)	RR cells (GFP+/RFP+), %	Nuclear Sox2 (IHC)	Estrogen receptor status
1	64.2%	13.9%	2+	+
2	48.7%	11.0%	2+	+
3	63.1%	16.1%	1+	+
4	57.8%	0.6%	N/A	-
5	43.0%	10.8%	N/A	+
6	49.7%	0.3%	3+	-
7	81.3%	12.5%	N/A	+
8	77.3%	21.4%	1+	+
9	36.3%	0.4%	N/A	-
10	61.0%	11.5%	N/A	+
11	- *	5.7%	0	+
12	- *	5.8%	3+	+
13	- *	17.0%	N/A	+
14	- *	21.6%	N/A	+
15	- *	23.8%	3+	+
16	- *	19.6%	2+	+
17	-*	5.4%	N/A	N/A
18	-*	10.5%	N/A	N/A
19	-*	5.8%	N/A	N/A

Flow cytometry analyses of red fluorescent protein (RFP) and green fluorescent protein (GFP) in mCMV-GFP-EF1-Puro infected primary breast tumor cells to set the gate thresholds and the SRR2-mCMV-GFP-EF1-RFP infected primary breast tumor cells showing % RFP+ cells (lentivirus infection efficiency) and % RFP+ GFP+ cells (% of patient reporter responsive (RR) cells). Table summarizes data from 19 primary breast tumor samples. The asterisks denote samples where the primary breast tumor cells % RFP+ could not be accurately assessed due to technical issues but is estimated to be approximately 60%. IHC denotes immunohistochemistry; N/A, not analyzed.

ISSN: 1465-542X