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Figure 1 | Breast Cancer Research

Figure 1

From: Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome-wide promoter microarrays

Figure 1

Sex-determining region Y (SRY)-box binding protein-2 (Sox2) occupies distinct promoter regions in reporter unresponsive (RU) and reporter responsive (RR) breast cancer cells. (A) Venn diagram of MCF7 RU and RR cells Sox2 chromatin immunoprecipitation promoter microarray chip analysis (ChIP-chip) study summarizing gene promoters bound by Sox2. (B) Functional annotation of MCF7 RU and RR putative Sox2 target genes with >2.0 peak score signal (compared to input DNA) using Protein Analysis THrough Evolutionary Relationships (PANTHER) Protein Class system. (C) MCF7 RU and RR ChIP DNA agarose gel results of DNA sequences immunoprecipitated by normal rabbit IgG or a rabbit anti-human Sox2 antibody amplified by GPR49 and MUC15 promoter specific primers. MCF7 RU and RR input represent the DNA isolated from chromatin before immunoprecipitation to show equal input amounts. Quantitative-PCR analyses of ChIP DNA derived from the IgG and Sox2 ChIP of MCF7 RU and RR cells using promoter-specific primers. Sox2 ChIP-qPCR signal was normalized to IgG signal as well as the respective RU and RR input signal.

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Breast Cancer Research

ISSN: 1465-542X

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